Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform.
نویسندگان
چکیده
Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Illumina TruSeq Exome Enrichment platform, which captures ∼62 Mb of the human exonic regions using 95-base DNA probes. In addition to covering the RefSeq and Ensembl coding sequences, the enriched sequences also include ∼28 Mb of RefSeq untranslated regions (UTR). The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.
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ورودعنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2015 7 شماره
صفحات -
تاریخ انتشار 2015